Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example.
Identifieur interne : 000020 ( Main/Exploration ); précédent : 000019; suivant : 000021Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example.
Auteurs : Eu Sheng Wang [Suède] ; Nam Phuong Kieu [Suède] ; Marit Lenman [Suède] ; Erik Andreasson [Suède]Source :
- Plants (Basel, Switzerland) [ 2223-7747 ] ; 2020.
Abstract
Potato (Solanum tuberosum) is among the best producers of edible biomass in terms of yield per hectare and a variety of different regional cultivars are used as a staple commodity in many countries. However, this crop is attacked by several diseases, with the worst being the late blight disease caused by Phytophthora infestans. Stacking of resistance (R) genes from wild Solanum relatives are interesting prospects for the sustainable control of late blight. Therefore, we optimized methods for the efficient generation and screening of R-gene-containing transformants in tetraploid and diploid hybrid potato genotypes. Using these methods, a high transformation efficiency was achieved for the transformation of tetraploid and diploid potato lines with a triple resistance (3R) gene construct. Transformation efficiencies were improved by optimizing several factors affecting regeneration, including the quality of the starting plant material, and the composition of the plant growth regulators used during selective regeneration. A refreshment protocol was designed to alleviate in vitro related stress in stock plants, which significantly improved the growth vigor and resulted in a 4- to 10-fold increase in transformation efficiency. Furthermore, long-term exposure to exogenous Indole-3-butyric acid that is usually used for the initiation of roots in vitro, was found to cause aberrant morphological phenotypes in potato.
DOI: 10.3390/plants9060695
PubMed: 32486039
PubMed Central: PMC7356882
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Kieu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Protection Biology, Swedish University of Agricultural SciencesSE, -230 53 Alnarp, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Plant Protection Biology, Swedish University of Agricultural SciencesSE, -230 53 Alnarp</wicri:regionArea><wicri:noRegion>-230 53 Alnarp</wicri:noRegion></affiliation></author><author><name sortKey="Lenman, Marit" sort="Lenman, Marit" uniqKey="Lenman M" first="Marit" last="Lenman">Marit Lenman</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Protection Biology, Swedish University of Agricultural SciencesSE, -230 53 Alnarp, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Plant Protection Biology, Swedish University of Agricultural SciencesSE, -230 53 Alnarp</wicri:regionArea><wicri:noRegion>-230 53 Alnarp</wicri:noRegion></affiliation></author><author><name sortKey="Andreasson, Erik" sort="Andreasson, Erik" uniqKey="Andreasson E" first="Erik" last="Andreasson">Erik Andreasson</name><affiliation wicri:level="1"><nlm:affiliation>Department of Plant Protection Biology, Swedish University of Agricultural SciencesSE, -230 53 Alnarp, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Plant Protection Biology, Swedish University of Agricultural SciencesSE, -230 53 Alnarp</wicri:regionArea><wicri:noRegion>-230 53 Alnarp</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plants (Basel, Switzerland)</title><idno type="ISSN">2223-7747</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Potato (<i>Solanum tuberosum</i>) is among the best producers of edible biomass in terms of yield per hectare and a variety of different regional cultivars are used as a staple commodity in many countries. However, this crop is attacked by several diseases, with the worst being the late blight disease caused by <i>Phytophthora infestans</i>. Stacking of resistance (R) genes from wild <i>Solanum</i> relatives are interesting prospects for the sustainable control of late blight. Therefore, we optimized methods for the efficient generation and screening of R-gene-containing transformants in tetraploid and diploid hybrid potato genotypes. Using these methods, a high transformation efficiency was achieved for the transformation of tetraploid and diploid potato lines with a triple resistance (3R) gene construct. Transformation efficiencies were improved by optimizing several factors affecting regeneration, including the quality of the starting plant material, and the composition of the plant growth regulators used during selective regeneration. A refreshment protocol was designed to alleviate in vitro related stress in stock plants, which significantly improved the growth vigor and resulted in a 4- to 10-fold increase in transformation efficiency. Furthermore, long-term exposure to exogenous Indole-3-butyric acid that is usually used for the initiation of roots in vitro, was found to cause aberrant morphological phenotypes in potato.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32486039</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Print">2223-7747</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><Issue>6</Issue><PubDate><Year>2020</Year><Month>May</Month><Day>29</Day></PubDate></JournalIssue><Title>Plants (Basel, Switzerland)</Title><ISOAbbreviation>Plants (Basel)</ISOAbbreviation></Journal><ArticleTitle>Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E695</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/plants9060695</ELocationID><Abstract><AbstractText>Potato (<i>Solanum tuberosum</i>) is among the best producers of edible biomass in terms of yield per hectare and a variety of different regional cultivars are used as a staple commodity in many countries. However, this crop is attacked by several diseases, with the worst being the late blight disease caused by <i>Phytophthora infestans</i>. Stacking of resistance (R) genes from wild <i>Solanum</i> relatives are interesting prospects for the sustainable control of late blight. Therefore, we optimized methods for the efficient generation and screening of R-gene-containing transformants in tetraploid and diploid hybrid potato genotypes. Using these methods, a high transformation efficiency was achieved for the transformation of tetraploid and diploid potato lines with a triple resistance (3R) gene construct. Transformation efficiencies were improved by optimizing several factors affecting regeneration, including the quality of the starting plant material, and the composition of the plant growth regulators used during selective regeneration. A refreshment protocol was designed to alleviate in vitro related stress in stock plants, which significantly improved the growth vigor and resulted in a 4- to 10-fold increase in transformation efficiency. Furthermore, long-term exposure to exogenous Indole-3-butyric acid that is usually used for the initiation of roots in vitro, was found to cause aberrant morphological phenotypes in potato.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Eu Sheng</ForeName><Initials>ES</Initials><AffiliationInfo><Affiliation>Department of Plant Protection Biology, Swedish University of Agricultural SciencesSE, -230 53 Alnarp, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kieu</LastName><ForeName>Nam Phuong</ForeName><Initials>NP</Initials><AffiliationInfo><Affiliation>Department of Plant Protection Biology, Swedish University of Agricultural SciencesSE, -230 53 Alnarp, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lenman</LastName><ForeName>Marit</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Plant Protection Biology, Swedish University of Agricultural SciencesSE, -230 53 Alnarp, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Andreasson</LastName><ForeName>Erik</ForeName><Initials>E</Initials><Identifier Source="ORCID">0000-0003-0666-7204</Identifier><AffiliationInfo><Affiliation>Department of Plant Protection Biology, Swedish University of Agricultural SciencesSE, -230 53 Alnarp, Sweden.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>mistra biotech</GrantID><Agency>Stiftelsen för Miljöstrategisk Forskning</Agency><Country></Country></Grant><Grant><GrantID>2015</GrantID><Agency>Svenska Forskningsrådet Formas</Agency><Country></Country></Grant><Grant><GrantID>2015</GrantID><Agency>Stiftelsen Lantbruksforskning</Agency><Country></Country></Grant><Grant><GrantID>CTS19</GrantID><Agency>Carl Tryggers Stiftelse för Vetenskaplig Forskning</Agency><Country></Country></Grant><Grant><GrantID>VR-reseach links</GrantID><Agency>Vetenskapsrådet</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>05</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Plants (Basel)</MedlineTA><NlmUniqueID>101596181</NlmUniqueID><ISSNLinking>2223-7747</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agrobacterium transformation</Keyword><Keyword MajorTopicYN="N">Phytophthora infestans</Keyword><Keyword MajorTopicYN="N">recalcitrant</Keyword><Keyword MajorTopicYN="N">refreshment protocol</Keyword><Keyword MajorTopicYN="N">resistance genes</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>04</Month><Day>24</Day></PubMedPubDate><PubMedPubDate 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uniqKey="Kieu N" first="Nam Phuong" last="Kieu">Nam Phuong Kieu</name><name sortKey="Lenman, Marit" sort="Lenman, Marit" uniqKey="Lenman M" first="Marit" last="Lenman">Marit Lenman</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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